| A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP |
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| 引用本文： | ZHANG Zhu-jun,ZHANG Su-sen and ZHANG Xin-rong (Department of Chemistry,Shaanxi Normal University,Xian,710062). A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP[J]. 高等学校化学研究, 1993, 0(4) |
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| 作者姓名： | ZHANG Zhu-jun ZHANG Su-sen and ZHANG Xin-rong (Department of Chemistry Shaanxi Normal University Xian 710062) |
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| 作者单位： | Department of Chemistry，Shaanxi Normal University，Xian，710062 |
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| 基金项目： | Supported by the National Natural Science Foundation of China |
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| 摘 要： | A new chemiluminescence amplification reaction has been developed, which is on the coupling of the oxidation of Eosin by hydrogen peroxide under the catalysis of HRP at pH 3. 5 to the chemiluminescent reaction of luminol and hydrogen peroxide catalyzed by HRP at pH 10 to measure the activity of HRP and labelled-HRP. The detection limit of HRP is 10-10 g/mL. The lowest dilution ratio of HBsAb-HRP is 1 : 1. 8×102 and 1 : 106 for the direct method and coupled method respectively.
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A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP |
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| ZHANG Zhu-jun,ZHANG Su-sen and ZHANG Xin-rong. A New Chemiluminescence Amplification Reaction for the Determination of HRP and Labelled HRP[J]. Chemical Research in Chinese University, 1993, 0(4) |
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| Authors: | ZHANG Zhu-jun ZHANG Su-sen ZHANG Xin-rong |
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| Abstract: | |
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| Keywords: | Chemiluminescence HRP Labelled HRP Determination |
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