| 鼠肝蛋白质组的纳升级多维液相色谱分离 |
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| 引用本文： | 王彦,高明霞,谷雪,张祥民. 鼠肝蛋白质组的纳升级多维液相色谱分离[J]. 色谱, 2005, 23(1): 41-45 |
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| 作者姓名： | 王彦 高明霞 谷雪 张祥民 |
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| 作者单位： | Department of Chemistry, Fudan University, Shanghai 200032, China |
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| 基金项目： | 国家自然科学基金资助项目(No.20475011),国家"863"计划(No.2002AA2Z2042),人类重大疾病蛋白组学研究项目(No.2001CB5102). |
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| 摘 要： | 搭建了一个纳升级的二维液相色谱分离平台(nano-2D-LC),该平台可以自动完成进样、除盐、分离及鉴定。以离子交换色谱(SCX)为第一维,反相液相色谱(RPLC)为第二维,对鼠肝组织的蛋白质组进行了研究。SCX采用阶梯式洗脱,RPLC运用线性梯度洗脱,以200 nL/min的速度进行分离,峰容量可达620。
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| 关 键 词： | 蛋白质组 多维液相色谱 纳升级 鼠肝 |
| 文章编号： | 1000-8713(2005)01-0041-05 |
| 收稿时间： | 2004-06-23 |
| 修稿时间： | 2004-09-30 |
Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver |
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| WANG Yan,GAO Mingxia,GU Xue,ZHANG Xiangmin. Nano Flow Multidimensional Liquid Chromatography for Proteome Analysis of Rat Liver[J]. Chinese journal of chromatography, 2005, 23(1): 41-45 |
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| Authors: | WANG Yan GAO Mingxia GU Xue ZHANG Xiangmin |
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| Affiliation: | Department of Chemistry, Fudan University, Shanghai 200032, China |
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| Abstract: | A nano flow comprehensive multidimensional liquid chromatography was developed. By using this separation platform, complex samples can be injected, desalted, separated and analyzed in complete automatization. Coupling of the strong cation-exchange (SCX) with capillary reversed-phase liquid chromatography (cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC. The flow rate through the nano scale analytical column during the separation process was maintained at 200 nL/min. The peak capacity for the nano-2D-LC was up to 620 under these conditions. |
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| Keywords: | proteomics multidimensional liquid chromatography rat liver nano flow |
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