Directed Evolution Methods for Enzyme Engineering |
| |
Authors: | Saurabh Rajendra Nirantar |
| |
Institution: | Illumina Singapore Inc., Singapore 757716, Singapore; |
| |
Abstract: | Enzymes underpin the processes required for most biotransformations. However, natural enzymes are often not optimal for biotechnological uses and must be engineered for improved activity, specificity and stability. A rich and growing variety of wet-lab methods have been developed by researchers over decades to accomplish this goal. In this review such methods and their specific attributes are examined. |
| |
Keywords: | directed evolution enzyme engineering protein engineering high throughput screening |
|