Interfacing ASE and HPLC for the Determination of PAH in Soil

A method is developed to couple an accelerated solvent extraction system (ASE) with high performance liquid chromatography (HPLC) for the analysis of PAH in soil samples. The resolution of HPLC is well maintained while the advantages of ASE, fast extraction, less solvent consumption and ease of operation,are well expressed. The precision and accuracy of this method are verified by a series of analysis of reference material and the precision of retention from multiple injection indicates minimum loss of chromatographic resolution by the interface of this technique. Detection limits for the studied PAH range from 0.07 ng/Kg to 0.21 ng/Kg with fluorescence detector.