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Simultaneous Capillary Electrophoretic Analysis of Flavonoids and Phenolic Acids in Plants
Authors:Kyoung Rae Kim  Saanglim Ryu  Sookie La  Jung Han Kim  Sangjun Lee
Affiliation:1. College of Pharmacy, Sungkyunkwan Univ. Suwon 440-746, Korea;2. Department of Biotechnology & Bioproducts Research Center, Yonsei University Seoul 120-749, Korea;3. Korea Research Institute of Bioscience and Biotechnology, KIST, Taejon 305-600, Korea
Abstract:An efficient capillary electrophoretic method is described for the simultaneous determination of hydroxylated and methoxylated flavonoids and phenolic acids present in plant extracts. The separation of samples injected in pressure injection mode was carried out using fused silica capillary in boric acid buffer (alkaline pH) containing SDS as pseudostationary phase and methanol as organic modifier with direct UV detection at 220nm.
Keywords:
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