Rapid Screening and Identification of Brefeldin A in Endophytic Fungi Using HPLC-MS/MS |
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| Authors: | Meijuan Fang Jianfeng Wang Yaojian Huang Yufen Zhao |
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| Affiliation: | (1) College of Chemistry and Engineering, Xiamen University, Xiamen, 361005, China;(2) School of Life Science, Xiamen University, Xiamen, 361005, China;(3) The Key Laboratory for Bioorganic Phosphorus Chemistry, Ministry of Education, Department of Chemistry School of Life Science and Engineering, Tsinghua University, Beijing, 100084, China |
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| Abstract: | Brefeldin A (BFA; C16H24O4, MW 280), a naturally occurring macrolide, exhibits diverse biological activities, such as antibiotic, antiviral, cytostatic, antimitotic, and antitumor effects. Owing to the wide activities of BFA, there is a need to develop a method for rapid identification of BFA in crude microbial extracts. In this paper, a method has been established and validated for screening and identification of individual as well as total BFA by high-performance liquid chromatography and liquid chromatography/electrospray mass spectrometry in fungal raw materials. Translated from Journal of Instrumental Analysis, 2005, 24(1) (in Chinese) |
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| Keywords: | HPLC-MS/MS endophytic fungi Brefeldin A antitumor |
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