| 基于磁性分离-硫化铜纳米粒子标记的DNA流动注射化学发光检测 |
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| 引用本文： | 朱金坤,任娟娟,徐颖,赵辉,何品刚,方禹之. 基于磁性分离-硫化铜纳米粒子标记的DNA流动注射化学发光检测[J]. 分析科学学报, 2009, 25(2) |
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| 作者姓名： | 朱金坤 任娟娟 徐颖 赵辉 何品刚 方禹之 |
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| 作者单位： | 华东师范大学化学系,上海,200062;周口师范学院化学系,河南周口,466000 |
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| 基金项目： | 国家自然科学基金，上海市浦江人才计划 |
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| 摘 要： | 本文基于磁性粒子(MB)良好的分离、富集能力,研究了硫化铜纳米粒子标记的流动注射-化学发光(FI-CL)DNA检测体系.通过硫化铜标记的探针1与目标DNA及连有磁球的探针2形成三明治结构,实现对目标DNA的捕获、分离与标记;通过其溶解释放出CuS标记颗粒的铜离子,引起化学发光信号增强,实现了目标DNA序列的定性定量检测.该方法对完全互补单链DNA(ssDNA)检测的线性范围为1.0×10-11～1.6×10-9 mol/L,检出限为3.0×10-12 mol/L,对1.0×10-9 mol/L目标DNA测定的相对标准偏差为3.2%(n=11),对目标碱基序列具有良好的识别能力.
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| 关 键 词： | 磁性分离 硫化铜 流动注射 化学发光 DNA |
Magnetic Separation based Flow Injection Chemiluminescent DNA Analysis with Copper Sulfide Labels |
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| ZHU Jin-kun,REN Juan-juan,XU Ying,ZHAO Hui,HE Pin-gang,FANG Yu-zhi. Magnetic Separation based Flow Injection Chemiluminescent DNA Analysis with Copper Sulfide Labels[J]. Journal of Analytical Science, 2009, 25(2) |
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| Authors: | ZHU Jin-kun REN Juan-juan XU Ying ZHAO Hui HE Pin-gang FANG Yu-zhi |
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| Affiliation: | 1.Chemistry Department;East China Normal University;Shanghai 200062;2.Chemistry Department;Zhoukou Normal University;Zhoukou 466000 |
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| Abstract: | Based on the super separation and enrichment ability of magnetic beads(MBs),we proposed a new flow injection chemiluminescence(FI-CL) analytical system for DNA detection by using CuS nanoparticles as tags.The assay relies on the hybridization of target DNA with probe 1(CuS-ssDNA conjugate) and probe 2(MB-ssDNA conjugate) to form a sandwich structure.The hybridization events were then monitored by the CL intensity catalyzed by the resolved Cu2+ ions from these CuS nanoparticles anchored on sandwich hybrids.U... |
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| Keywords: | DNA |
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