| 细菌视紫红质（BR）的表面光电压谱研究 |
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| 引用本文： | 王宝辉,王今堆.细菌视紫红质（BR）的表面光电压谱研究[J].光谱学与光谱分析,1997,17(2):35-40. |
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| 作者姓名： | 王宝辉 王今堆 |
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| 作者单位： | [1]大庆石油学院石油化工系 [2]吉林大学分子生物系 |
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| 摘 要： | 采用表面光电压谱系统地研究了细菌视紫红质的光伏特性，结果表明ＢＲ薄膜有着独特的光电性质，并且与ＢＲ薄膜的制备方法和湿度有关，从经过ＨＥＰＥＳ修饰的ＢＲ表面光电压谱，观察到了ＢＲ的中间体Ｍ４１２和Ｏ６４０响应峰。说明ＨＥＰＥＳ对ＢＲ中间体的稳定作用，这些现象可由ＢＲ的光循环和光电压产生机理来解释。
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| 关 键 词： | 细菌视紫红质 表面光电压谱 光电响应 |
SURFACE PHOTOVOLTAIC SPECTROSCOPIC INVESTIGATION OF BACTERIORHODOPSIN(BR) |
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| B Wang,J Wang,X Fu,D Wang,Y Cui,T Li.SURFACE PHOTOVOLTAIC SPECTROSCOPIC INVESTIGATION OF BACTERIORHODOPSIN(BR)[J].Spectroscopy and Spectral Analysis,1997,17(2):35-40. |
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| Authors: | B Wang J Wang X Fu D Wang Y Cui T Li |
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| Institution: | Department of Petrochemical Engineering, Daqing petroleum Institute, 151400 ANda. |
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| Abstract: | The surface photovoltaic spectra(SPS) of Bacteriorhodopsin based films were systematically measured. The results show that the BR films are characterized by the specific photovoltaic effect. The SPS response of the BR films depends on the fabrication and humidity of the film. The intermediate M 412 and O 640 of BR are observed in the SPS of HEPES containing BR film,indicating that HEPES has very important function to stabilize intermediate M 412 . The observations are explained in terms of the photocycle of BR and the mechanism of photovoltage generation. |
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| Keywords: | Bacteriorhodopsin Surface photovoltaic spectroscopy Photoelectric effect |
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