Polarographic determination of cyanide as nickelcyano complex in blood plasma after selective extraction in a methylene blue impregnated polyethylene column |
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Authors: | D Bohrer P C do Nascimento S Garcia Pomblum E Seibert L Machado de Carvalho |
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Institution: | Department of Chemistry, Federal University of Santa Maria, BR-97111-970 Santa Maria, RS Brazil, BR
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Abstract: | A method for the determination of cyanide in blood plasma by differential pulse polarography (DPP) is described without a drastic acidification of the sample. Cyanide was determined as tetracyanonickelate(II)-anion complex after a microwave-acid assisted cleanup and a selective complex extraction in a polyethylene methylene blue (PE-MB) impregnated column. The cyano complex was eluted from the column with water/acetonitrile and determined by pulse-polarography at –380 mV (Ag/AgCl). The linear range of calibration was obtained from 1.2 to 9.6 μg of cyanide with r = 0.99 and RSD = 9% of 1.2 μg of cyanide. A detection limit of 40 μg L–1 was calculated and the recoveries of cyanide from spiked samples were about 80%. This method was compared with the classical pyridine-pyrazolone method. |
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