| 高效阴离子交换色谱-铜/铂修饰电极安培法测定大观霉素 |
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| 引用本文： | 任丹丹,习玲玲,黄伟民,朱岩. 高效阴离子交换色谱-铜/铂修饰电极安培法测定大观霉素[J]. 分析化学, 2010, 38(11). DOI: 10.3724/SP.J.1096.2010.01563 |
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| 作者姓名： | 任丹丹 习玲玲 黄伟民 朱岩 |
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| 作者单位： | 浙江大学西溪校区化学系,杭州,310028;浙江大学西溪校区化学系,杭州,310028;浙江大学西溪校区化学系,杭州,310028;浙江大学西溪校区化学系,杭州,310028 |
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| 基金项目： | 国家自然科学基金(No.20775070); 浙江省自然科学基金(No.4100190)资助 |
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| 摘 要： | 采用阴离子交换色谱分离,在CuSO4溶液中加入少量镧系化合物,经电沉积制备La3+-Cu/pt/CME工作电极,建立了直流安培电化学法(DC)直接检测硫酸大观霉素的方法。考察了流动相浓度、测定电位等参数对色谱分离和测定的影响。在固定相为CarboPac PA10阴离子交换柱、流动相为26mmol/L NaOH,流速为0.6mL/min的色谱条件下,检测电位为0.68V时,硫酸大观霉素峰面积与其浓度在0.12～12mg/L(r=0.9991)和12～280mg/L(r=0.9995)两个范围内呈线性。本方法不需要柱前和柱后衍生化,能同时测定硫酸大观霉素中的主要组分和杂质。修饰电极制作方法简单,催化稳定性好,可作为电化学传感器测定硫酸大观霉素中的各组分。
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| 关 键 词： | 铜/铂化学修饰电极 高效阴离子交换色谱 直流安培法 大观霉素 |
Copper Modified Platinum Electrode for Amperometric Detection of Spectinomycin Sulfate by Anion Exchange Chromatography |
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| REN Dan-Dan,XI Ling-Ling,HUANG Wei-Min,ZHU Yan. Copper Modified Platinum Electrode for Amperometric Detection of Spectinomycin Sulfate by Anion Exchange Chromatography[J]. Chinese Journal of Analytical Chemistry, 2010, 38(11). DOI: 10.3724/SP.J.1096.2010.01563 |
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| Authors: | REN Dan-Dan XI Ling-Ling HUANG Wei-Min ZHU Yan |
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| Affiliation: | REN Dan-Dan,XI Ling-Ling*,HUANG Wei-Min,ZHU Yan ( Department of Chemistry,Xixi Campus,Zhejiang University,Hangzhou 310028) |
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| Abstract: | A La3+ -Cu/Pt modified electrode was fabricated by electrodepositing process in CuSO4 solution by adding a small amount of lanthium compound,and was employed for direct current ( DC) amperometric detection of spectinomycin by anion-exchange chromatography. Experimental conditions such as detection potential, concentration of mobile phase on chromatographic resolution and detection sensitivity were evaluated. The column was CarboPac PA10,the mobile phase was 26 mmol/L NaOH solution,the flow rate of mobile ph... |
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| Keywords: | Copper/platinum chemically modified electrode High performance anion exchange chromatography Direct current amperometric detection Spectinomycin |
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