Component Analysis and Anti-Colorectal Cancer Mechanism via AKT/mTOR Signalling Pathway of Sanghuangporus vaninii Extracts |
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| Authors: | Shanshan Guo Wenwen Duan Yaxin Wang Liangmian Chen Chenchen Yang Xuezhu Gu Qinghai Xue Raorao Li Zhijie Zhang |
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| Institution: | 1.Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China; (S.G.); (W.D.); (Y.W.); (L.C.); (C.Y.); (X.G.);2.School of Pharmaceutical Sciences, Zhengzhou University, Zhengzhou 450001, China;3.The TCM Clinic of China Academy, China Academy of Chinese Medical Sciences, Beijing 100700, China; |
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| Abstract: | Sanghuangporus vaninii (Ljub.) L.W. Zhou & Y.C. Dai (SV) is a major cultivar of Sanghuang, which is well known as an excellent anti-tumour drug and reaches the mainstream market in China. Water, 60% ethanol and 95% ethanol were used to extract the drug, and three kinds of polar extracts were obtained separately. Compared with water extracts and 95% ethanol extracts, the 60% ethanol extract had the highest flavonoid content, and its polysaccharide content was greater than that in the 95% ethanol extract and lower than that in the water extract. Its essential components were phenolics whose majority were phenolic acids, flavonoids and phenylpropanoids. This extract has better inhibition effects on the proliferation of SW480 human colon cancer cells, inducing cell apoptosis and blocking G2/M period cells. It can significantly inhibit gene expression and reduce the activation of the AKT/mTOR signalling pathway. The anti-cancer activity of the 60% ethanol extract is satisfactory and may be a result of the combined effects of polysaccharides and flavonoids. The data suggest that the 60% ethanol extract can be used as an adjuvant for chemotherapy and as a potential anti-cancer agent with broad development prospects. |
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| Keywords: | Sanghuangporus vaninii (Ljub ) L W Zhou & Y C Dai (SV) extracts component analysis colon cancer AKT/mTOR |
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