Yield optimization in the kinetically controlled enzymic peptide synthesis |
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| Affiliation: | 1. Instituto de Pesquisas Energéticas e Nucleares (IPEN/CNEN-SP) ⿿ Av. Prof. Lineu Prestes, 2242, Cidade Universitária, 05508-000 São Paulo, SP, Brazil;2. Institute of Applied Radiation Chemistry, Lodz University of Technology, Wroblewskiego 15, 93-590, Lodz, Poland;1. College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, PR China;2. College of Pharmaceutical Engineering and Science, Zhejiang Chinese Medical University, Hangzhou 310036, PR China;3. Department of Energy, Environmental and Chemical Engineering, Washington University, St. Louis, MO 63130, USA;1. Department of Informatics, Bioengineering, Robotics and Systems Engineering, University of Genoa, Via all’Opera Pia 13, 16145 Genoa, Italy;2. Nanoscopy and NIC, Istituto Italiano di Tecnologia, Via Morego 30 16163, Genoa, Italy;3. Department of Chemistry, Materials and Chemical Engineering ‘G. Natta’, UdR INSTM Milano Politecnico, Politecnico di Milano, Piazza Leonardo da Vinci 32, 20133 Milan, Italy;4. Department of Chemistry and Industrial Chemistry, University of Genoa, Via Dodecaneso 31, 16146, Genoa, Italy |
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| Abstract: | The yield and its time-dependence in acylenzyme mechanism-based enzymic peptide synthesis are controlled by the proteinase kinetic specificity. The maximum yield is limited by a non-equilibrium constant Kmax. Both Kmax and the time, tmax, taken to attain the maximum yield, are directly related to the enzyme kinetic parameters. These relationships allow kinetic determination of yield optimization in kinetically controlled enzymic peptide synthesis. |
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