A high-resolution phosphorus-31 nuclear magnetic resonance (NMR) spectroscopic method for the non-phosphorus markers of chemical warfare agents |
| |
Authors: | Mazumder Avik Kumar Ajeet Purohit Ajay K Dubey Devendra K |
| |
Institution: | (1) Vertox Laboratory, Defence Research and Development Establishment, Jhansi Road, Gwalior, 474002, India; |
| |
Abstract: | A high-resolution phosphorus-31 nuclear magnetic resonance (NMR) spectroscopic method has been developed for detection, identification
and quantification of non-phosphorus markers of toxic nerve agents (soman and V-class), vesicants (HD, HN-2, HN-3), and incapacitating agent (Bz). These analytes were converted to phosphorus-containing
derivatives via phosphitylation reaction of their hydroxyl and sulfhydryl functions (using 2-chloro-4,4,5,5-tetramethyl-1,3,2-dioxaphospholane).
This was followed by 31P{1H} and 31P NMR analysis of these derivatives. The chemical shifts (δ) and coupling constants (3
J
P–H) of derivatives were used for their specific detection and identification. The method allowed clear distinction between the
alcohols and thiols. The lower limits of detection of these analytes were found to be between 12 and 28 μg obtained from 128
transients of 31P{1H} quantitative NMR experiments. Utility of the method was ensured by the detection and identification of triethanolamine
present (at an original concentration of 5 μg/mL) in an aqueous sample from 28th OPCW Official Proficiency Tests. |
| |
Keywords: | |
本文献已被 PubMed SpringerLink 等数据库收录! |
|