Interference of flavonoids with enzymatic assays for the determination of free fatty acid and triglyceride levels |
| |
| Authors: | Email author" target="_blank">Elise?F?Hoek-van den HilEmail author Karsten?Beekmann Jaap?Keijer Peter?C?H?Hollman Ivonne?M?C?M?Rietjens Evert?M?van?Schothorst |
| |
| Institution: | (1) Division of Toxicology, Wageningen University, Tuinlaan 5, 6703 HE Wageningen, The Netherlands;(2) Division of Human and Animal Physiology, Wageningen University, De Elst 1, 6708 WD Wageningen, The Netherlands;(3) RIKILT-Institute of Food Safety, Akkermaalsbos 2, 6708 WB Wageningen, The Netherlands |
| |
| Abstract: | Flavonoids are bioactive food compounds with potential lipid-lowering effects. Commercially available enzymatic assays are
widely used to determine free fatty acid (FFA) and triglyceride (TG) levels both in vivo in plasma or serum and in vitro in
cell culture medium or cell lysate. However, we have observed that various flavonoids interfere with peroxidases used in these
enzymatic assays, resulting in incorrect lower FFA and TG levels than actually present. Furthermore, addition of isorhamnetin
or the major metabolite of the flavonoid quercetin in human and rat plasma, quercetin-3-O-glucuronide, to murine serum also resulted in a significant reduction of the detected TG levels, while a trend was seen for
FFA levels. It is concluded that when applying these assays, vigilance is needed and alternative analytical methods, directly
assessing FFA or TG levels, should be used for studying the biological effects of flavonoids on FFA and TG levels. |
| |
| Keywords: | |
| 本文献已被 PubMed SpringerLink 等数据库收录！ |
|
