Probe functionalization with a Rhop-3 antibody: toward a Rhop-3 antigen immunosensor for detection of malaria |
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Authors: | Salaam?Saleh Susan?Moreno-Molek Indika?Perera Alan?Riga Tobili?Sam-Yellowe Email author" target="_blank">Mekki?BayachouEmail author |
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Institution: | (1) Department of Biological, Geological, and Environmental Sciences, College of Science and Health Professions, Cleveland State University, Cleveland, OH 44115, USA;(2) Department of Chemistry, College of Science and Health Professions, Cleveland State University, Cleveland, OH 44115, USA;(3) Department of Pathobiology (NC22), Lerner Research Institute, Cleveland Clinic, 9500 Euclid Ave, Cleveland, OH 44195, USA; |
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Abstract: | The antibody specific for the malaria protein, Rhop-3, and FL-Rhop-3, were immobilized on the surface of a gold electrode
modified with cysteamine. Colloidal gold was used to enhance the detection signal for Rhop-3 antigens. The Rhop-3 antibody
was also immobilized on gold electrodes preactivated with dithiobis(succinimidyl proprionate) (DSP). Immobilization was performed
at room temperature and at 37 °C. Cyclic voltammetry (CV) was used to monitor the interaction between the immobilized antibody
and its cognate antigen in solution, using ferricyanide, K3Fe(CN)6, as reporting electroactive probe. Tests indicate recognition of Rhop-3 protein by the immobilized antibody. Antigen recognition
was enhanced by incubation at 37 °C compared with room-temperature incubation. Our results suggest that an immunosensor can
be developed and optimized to aid detection of Rhop-3 antigens in samples from malaria patients. As far as we are aware, this
is the first amperometric immunosensor targeting Rhop-3 antigen as a malaria biomarker. |
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