Functionalized gold nanoparticles for ultrasensitive DNA detection |
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Authors: | Laura Maria Zanoli Roberta D’Agata Giuseppe Spoto |
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Institution: | (1) Scuola Superiore di Catania, c/o Dipartimento di Scienze Chimiche, Universit? di Catania, Viale Andrea Doria 6, 95125 Catania, Italy;(2) Dipartimento di Scienze Chimiche, Universit? di Catania, Viale Andrea Doria 6, 95125 Catania, Italy;(3) Istituto Biostrutture e Bioimmagini, CNR, Viale A. Doria 6, 95125 Catania, Italy; |
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Abstract: | A major challenge in the area of DNA detection is the development of rapid methods that do not require polymerase chain reaction
(PCR) amplification of the genetic sample. The PCR amplification step increases the cost of the assay, the complexity of the
detection, and the quantity of DNA required for the assay. In this context, methods that are able to perform DNA analyses
with ultrasensitivity have recently been investigated with the aim of developing new PCR-free detection protocols. Functionalized
gold nanoparticles have played a central role in the development of such methods. Here, possibilities offered by functionalized
gold nanoparticle in the ultrasensitive detection of DNA are discussed. The different functionalization protocols available
for gold nanoparticles and the principal DNA detection methods that are able to detect DNA at the femtomolar to attomolar
level are presented. |
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Keywords: | |
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