Biochemical localization of a protein involved in synthesis of Gluconacetobacter hansenii cellulose |
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| Authors: | Prashanti R Iyer Jeffrey Catchmark Nicole R Brown and Ming Tien |
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| Institution: | (1) Graduate Program in Chemical Biology, Huck Institute of Life Sciences, The Pennsylvania State University, University Park, PA 16802, USA;(2) Department of Agricultural and Biological Engineering, The Pennsylvania State University, University Park, PA 16802, USA;(3) School of Forest Resources, The Pennsylvania State University, University Park, PA 16802, USA;(4) Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, PA 16802, USA; |
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| Abstract: | Using subcellular fractionation and Western blot methods, we have shown that AcsD, one of the proteins encoded by the Acetobacter
cellulose synthase (acs) operon, is localized in the periplasmic region of the cell. AcsD protein was heterologously expressed
in Escherichia coli and purified using histidine tag affinity methods. The purified protein was used to obtain rabbit polyclonal antibodies.
The purity of the subcellular fractions was assessed by marker enzyme assays. |
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