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Highly Sensitive Impedimetric Biosensor Based on Thermolysin Immobilized on a GCE Modified with AuNP-decorated Graphene for the Detection of Ochratoxin A |
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| Authors: | Dr. Khaoula Zinoubi Dr. Amani Chrouda Dr. Raya Soltane Prof. Dr. Youssef O. Al-Ghamdi Prof. Dr. Sami Garallah Almalki Prof. Dr. Gamal Osman Prof. Dr. Houcine Barhoumi Nicole Jaffrezic Renault |
| Affiliation: | 1. Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, University of Monastir, Monastir, Tunisia;2. Department Faculty of Sciences of Tunis, Tunis El Manar University, Tunisia Department of Basic Sciences, Adham University college, Umm Al-Qura University, Adham, 21971 Saudi Arabia;3. Department of chemistry, College of Science at Zulfi, Majmaah University, Zulfi, 11932 Saudi Arabia;4. Department of Biology, College of Science Al-zulfi, Majmaah University, Al-Majmaah, 11952 Saudi Arabia;5. Department of Biology, Faculty of Applied Sciences, Umm Al-Qura University, Makkah, Saudi Arabia. Research Laboratories Center, Faculty of Applied Science, Umm Al-Qura University, Mecca, Saudi Arabia Agricultural Genetic Engineering Research Institute (AGERI), ARC, 12619 Giza, Egypt;6. Laboratory of Interfaces and Advanced Materials, Faculty of Sciences, University of Monastir, Monastir, Tunisia Institute of Analytical Sciences, UMR CNRS-UCBL 5280, 5 Rue la Doua, 69100 Villeurbanne Cedex, France;7. Institute of Analytical Sciences, UMR CNRS-UCBL 5280, 5 Rue la Doua, 69100 Villeurbanne Cedex, France |
| Abstract: | The fabrication of a thermolysin-based biosensor capable of detecting ochratoxin A (OTA) from food samples is described. The electrochemical deposition of calcium cross-linked cellulose film (CCLC) and gold nanoparticles (AuNPs) on graphene (GR) for modification of a glassy carbon electrode (GCE) is the first step. Then the thermolysin (TLN) enzyme in a polyvinyl alcohol (PVA)/polyethylenimine (PEI) matrix is immobilized. The impedimetric biosensor response is linear from 0.2 nM to 100 nM with a detection limit of 0.2 nM. The obtained stable and reproducible biosensor is then applied for the detection of OTA in spiked extracts from coffee beans. |
| Keywords: | Ochratoxin A Thermolysin Electrochemical impedance spectroscopy Biosensor |