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Electrochemical Biosensor Design with Multi-walled Carbon Nanotube to Display DNA-Schiff Base Interaction |
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| Authors: | Azeema Munir Burcin Bozal-Palabiyik Gokcen Eren Afzal Shah Saqib Ali Noor Uddin Dr. Bengi Uslu |
| Affiliation: | 1. Quaid-i-Azam University, Department of Chemistry, 45320 Islamabad, Pakistan Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560 Ankara, Turkey;2. Ankara University, Faculty of Pharmacy, Department of Analytical Chemistry, 06560 Ankara, Turkey;3. Gazi University, Faculty of Pharmacy, Department of Pharmaceutical Chemistry, 06330 Etiler-Ankara, Turkey;4. Quaid-i-Azam University, Department of Chemistry, 45320 Islamabad, Pakistan |
| Abstract: | This paper demonstrates a Schiff base i. e. 5-(diethylamino)-2-((2,6-diethylphenylimino)methyl)phenol (5-DDMP) that was sensed by DNA biosensor. dsDNA was immobilized onto GCE modified with functionalized multi-walled carbon nanotubes to prepare a biosensor. The efficiency of dsDNA biosensor was determined and binding of 5-DDMP with dsDNA was searched by UV-vis spectrophotometry and differential pulse voltammetry. Molecular docking simulations between 5-DDMP and dsDNA were explored and as a result, a hydrogen bond and a π-π contact were observed between 5-DDMP and deoxyguanosine base (dG22) of the strand B, deoxyadenosine base (dA5) of the strand A, respectively. These studies could be useful for new anticancer drug design and development. |
| Keywords: | Schiff base DNA biosensor carbon nanotube voltammetry molecular docking |