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Hydrogen Peroxide Detection Using Prussian Blue-modified 3D Pyrolytic Carbon Microelectrodes |
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| Authors: | Sheida Esmail Tehrani Long Quang Nguyen Giulia Garelli Bettina M Jensen Tautgirdas Ruzgas Jenny Emnéus Stephan Sylvest Keller |
| Institution: | 1. National Centre for Nano Fabrication and Characterization (DTU Nanolab), Technical University of Denmark, Ørsteds Plads, Building 347, 2800 Kongens Lyngby, Denmark;2. Allergy Clinic, Copenhagen University Hospital at Herlev-Gentofte, Gentofte Hospitalsvej 8, 2900 Hellerup, Denmark;3. Biofilms Research Center for Biointerfaces, Department of Biomedical Science, Malmö University, Per Albin Hanssons väg 35, Forskaren Building, 21432 Malmö, Sweden;4. Department of Biotechnology and Biomedicine (DTU Bioengineering), Technical University of Denmark, Produktionstorvet, Building 423, 2800 Kongens Lyngby, Denmark |
| Abstract: | A highly sensitive amperometric Prussian blue-based hydrogen peroxide sensor was developed using 3D pyrolytic carbon microelectrodes. A 3D printed multielectrode electrochemical cell enabled simultaneous highly reproducible Prussian blue modification on multiple carbon electrodes. The effect of oxygen plasma pre-treatment and deposition time on Prussian blue electrodeposition was studied. The amperometric response of 2D and 3D sensors to the addition of hydrogen peroxide in μM and sub-μM concentrations in phosphate buffer was investigated. A high sensitivity comparable to flow injection systems and a detection limit of 0.16 μM was demonstrated with 3D pyrolytic carbon microelectrodes at stirred batch condition |
| Keywords: | Prussian blue hydrogen peroxide sensor amperometry pyrolytic carbon |