Neuraminidase Based Electro-Nano Diagnostic Platforms: Development of Model Systems for Cancer Diagnosis

An electrochemical biosensor that monitored neuraminidase (NEU3), activity was developed. The analysis platform included a graphene-platinum hybrid modified gold screen printed electrode as a transducer. The detection protocol was based on observation of NEU3 activity which was used to remove sialic acid from the GD3 ganglioside. Examination of analytical characteristics resulted with two linear ranges of 10⁻⁸ U/mL–10⁻¹ and 10⁻¹ U/mL–2.53 U/mL with limit of detection values of 10⁻⁸ U/mL and 10⁻¹ U/mL, respectively. The selectivity of the developed NEU3 activity based electrochemical biosensor was tested with HeLa, VERO and A549 cell lines.