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Capillary electrophoretic method for Staphylococcus aureus detection by using a novel antimicrobial peptide |
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| Authors: | Tingting Hong Lin Qiu Wen Rui Ying Li Ronghui Zheng Qianqian Guo Shuwen Zhou Pengfei Cui Jingyu Sheng Xinye Ni Jianhao Wang Pengju Jiang |
| Affiliation: | 1. School of Pharmacy, Changzhou University, Changzhou, Jiangsu, P. R. China;2. School of Pharmacy, Changzhou University, Changzhou, Jiangsu, P. R. China Jiangsu Science Standard Medical Testing Co. Ltd, Changzhou, Jiangsu, P. R. China;3. School of Pharmacy, Changzhou University, Changzhou, Jiangsu, P. R. China Jiangsu Traumark Medical Instrument Co. Ltd., Changzhou, Jiangsu, P. R. China;4. Wujin Hospital, Affiliated with Jiangsu University, Changzhou, Jiangsu, P. R. China;5. The Affiliated Changzhou No. 2 People's Hospital of Nanjing Medical University, Changzhou, Jiangsu, P. R. China |
| Abstract: | A novel peptide containing antimicrobial sequence and gelatinase cleavage sites was designed for Staphylococcus aureus detection. Since Staphylococcus aureus could secrete gelatinase, the fluorescein labeled peptide GKRWWKWWRRPLGVRGC could be recognized and cleaved. The obtained products were able to be analyzed by capillary electrophoresis with fluorescence detection. To explore the effect of Staphylococcus aureus concentration on enzyme digestion ability of peptide, Staphylococcus aureus with different concentrations were incubated with the peptide. Results indicated that capillary electrophoretic method was efficient for determining Staphylococcus aureus content. Compared with traditional approaches for Staphylococcus aureus detection, capillary electrophoresis possessed higher efficiency, enhanced sensitivity, and low sample consumption. Moreover, the proposed peptide also presented desirable antimicrobial activity. It suggested that the novel antimicrobial peptide used in this research opens a new path of detecting Staphylococcus aureus by capillary electrophoretic method. |
| Keywords: | Antimicrobial peptide Capillary electrophoresis Staphylococcus aureus |