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Development and validation of a forensic six-dye multiplex assay with 29 STR loci |
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| Authors: | Yiling Qu RuiYang Tao Huan Yu Qi Yang Ziwei Wang Rui Tan Xiaochun Zhang Ruocheng Xia Lei Xiong Shihan Xi Jun Wu Yuzhen Gao Suhua Zhang Chengtao Li |
| Institution: | 1. Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China;2. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China;3. Department of Forensic Science, Medical School of Soochow University, Suzhou, P. R. China
Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China;4. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China Department of Forensic Medicine, School of Basic Medical Science, Wenzhou Medical University, Wenzhou, P. R. China;5. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China School of Basic Medicine, Inner Mongolia Medical University, Hohhot, P. R. China;6. Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Academy of Forensic Sciences, Ministry of Justice, Shanghai, P. R. China Clinical Medical School of Inner Mongolia University for the Nationalities, Tongliao, P. R. China;7. PeopleSpot Inc., Beijing, P. R. China |
| Abstract: | This paper describes the development and validation of a novel 31-locus, six-dye STR multiplex system, which is designed to meet the needs of the rapidly growing Chinese forensic database. This new assay combines 20 extended-CODIS core loci (D3S1358, D5S818, TPOX, CSF1PO, TH01, vWA, D7S820, D21S11, D8S1179, D18S51, D16S539, D13S317, FGA, D1S1656, D2S441, D2S1338, D10S1248, D12S391, D19S433, and D22S1045), nine highly polymorphic loci in Chinese Han population (D3S3045, D6S1043, D6S477, D8S1132, D10S1435, D15S659, D19S253, Penta D, and Penta E), and two gender determining markers, amelogenin and Y-Indel, which could amplify DNA from extracts, as well as direct amplification from substrates. To demonstrate the suitability for forensic applications, this system was validated by precision and accuracy evaluation, concordance tests, case sample tests, sensitivity, species specificity, stability, stutter calculation, and DNA mixtures, according to the guidelines described by the Scientific Working Group on DNA Analysis Methods (SWGDAM) and regulations published by the China Ministry of Public Security. The validation results indicate the robustness and reliability of this new system, and it could be a potentially helpful tool for human identification and paternity testing in the Chinese population, as well as facilitating global forensic DNA data sharing. |
| Keywords: | Capillary electrophoresis Developmental validation Forensic genetics Multiplex PCR assay Short tandem repeats |