|
|||||
|
|
SECM for Studying the Immobilization and Repartition of a Redox Anti-tetracycline Aptamer on Screen-printed Carbon Electrodes |
|
| Authors: | Ahmad Alawad Laure Latapie David Evrard Pierre Gros Georges Istamboulie Thierry Noguer Carole Calas-Blanchard |
| Institution: | 1. Université de Perpignan Via Domitia, Biocapteurs-Analyse-Environnement, 66860 Perpignan, France
Laboratoire de Biodiversité et Biotechnologies Microbiennes, USR 3579 Sorbonne Universités (UPMC) Paris 6 et CNRS Observatoire Océanologique, 66650 Banyuls-sur-Mer, France;2. Laboratoire de Génie Chimique, Université de Toulouse, CNRS, INPT, UPS, Toulouse, France;3. Université de Perpignan Via Domitia, Biocapteurs-Analyse-Environnement, 66860 Perpignan, France |
| Abstract: | Scanning electrochemical microscopy (SECM) is discussed as a versatile tool to provide a unique approach of localized electrochemical information in the context of biosensing research. The step-by-step immobilization of DNA aptamer with intrinsic redox activity on screen-printed carbon electrode (SPCE) was successfully monitored using SECM imaging tool. Control experiments were performed with a non-electroactive aptamer. After immobilization of these aptamers, SECM images showed the repartition of the electroactive anti-tetracycline aptamer when comparing with images produced for control and for all modification steps of SPCE. The possibility of tetracycline detection was also proved by causing a decrease in recorded current. |
| Keywords: | Scanning electrochemical microscopy Electrochemical aptasensor Electroactive aptamer Tetracycline |