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Electroactivity of Superoxide Anion in Aqueous Phosphate Buffers Analyzed with Platinized Microelectrodes |
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| Authors: | Pauline Lefrançois Fanny Girard-Sahun Vasilica Badets Franck Clément Stéphane Arbault |
| Institution: | 1. Univ. Bordeaux, Bordeaux INP, CNRS UMR 5255, ISM, groupe NSysA, 33400 Talence, France
Department of Biochemistry, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Nijenborgh 4, 9747 AG, Groningen, The Netherlands These two authors contributed equally to the paper.;2. Univ. Bordeaux, Bordeaux INP, CNRS UMR 5255, ISM, groupe NSysA, 33400 Talence, France UPPA, IPREM, CNRS UMR 5254, 2 avenue Président Angot, 64000 Pau, France Chemistry Department, University of Antwerp, Campus Drie Eiken Universiteitsplein 1, Belgium. These two authors contributed equally to the paper.;3. Univ. Bordeaux, Bordeaux INP, CNRS UMR 5255, ISM, groupe NSysA, 33400 Talence, France University of Strasbourg, Chemistry Institute, UMR CNRS 7177, 4 rue Blaise Pascal, CS 90032, 67081 Strasbourg cedex, France;4. UPPA, IPREM, CNRS UMR 5254, 2 avenue Président Angot, 64000 Pau, France;5. Univ. Bordeaux, Bordeaux INP, CNRS UMR 5255, ISM, groupe NSysA, 33400 Talence, France |
| Abstract: | The reactivity of platinized ultramicroelectrodes (Pt-black UMEs) towards superoxide anion O2.−, an unstable Reactive Oxygen Species (ROS), and its relatives, H2O2 and O2, was studied. Voltammetric studies in PBS demonstrate that Pt-black UMEs provide: i) a well-resolved reversible redox signature for O2.− detected in both alkaline and physiological buffers (pH 12 and 7.4); ii) irreversible oxidation and reduction waves for H2O2 at pH 7.4. The oxygen reduction reaction (ORR) at Pt-black surfaces solely yields H2O2 (2 electrons/2 H+) at physiological pH. Consequently, Pt-black UMEs allow to sense different ROS including superoxide anion for future biomedical or physico-chemical investigations. |
| Keywords: | superoxide ultramicroelectrode hydrogen peroxide reactive oxygen species cold atmospheric plasma |