Electrochemical Aptasensor for Zearalenone Based on DNA Assembly and Exonuclease III as Amplification Strategy |
| |
| Authors: | Min Wei Lingkun Xin Huali Jin Yawei Huang Yong Liu |
| |
| Institution: | 1. College of Food Science and Technology, Henan Key Laboratory of Cereal and Oil Food Safety Inspection and Control, Henan University of Technology, Zhengzhou, 450001 PR China;2. College of Chemistry and Chemical Engineering, Henan University, Kaifeng, 475004 PR China |
| |
| Abstract: | A sensitively electrochemical aptasensor was developed to detect zearalenone, utilizing DNA assembly based on hybridization chain reaction to amplify the signal current and exonuclease III to reduce the background current. The linear range 5.0×10?5 ng/mL-50 ng/mL, and the limit of detection is 0.013 pg/mL. The fabricated aptasensor showed the high specificity toward aflatoxin B1 (AFB1), fumonisin B1 (FB1) and ochratoxin A (OTA), good repeatability and reproducibility. In addition, the average recoveries of spiked corn and beer samples were in the range of 89 % to 102 %. The established method is of great significance in the field of food safety detection. |
| |
| Keywords: | Electrochemical aptasensor DNA assembly exonuclease III zearalenone |
|
|
