Rapid Nanomolar Detection of Guaiacol from its Precursors Using a Core-shell Reversed-phase Column Coupled with a Boron-doped Diamond Electrode |
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| Authors: | Phyllis E Hayes Alyah Buzid John H T Luong Jeremy D Glennon |
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| Institution: | 1. Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), School of Chemistry and the Analytical & Biological Chemistry Research Facility (ABCRF), University College Cork, College Road, Cork, T12 YN60 Ireland;2. Department of Chemistry, College of Science, King Faisal University, P.O. Box 380, Al-Ahsa, 31982 Saudi Arabia. |
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| Abstract: | The electrooxidation mechanisms of guaiacol (2-methoxyphenol), a food and beverage spoilage metabolite, and its precursors; vanillic acid (VA), vanillin, and ferulic acid (FA) were investigated by cyclic voltammetry (CV) with a boron-doped diamond (BDD) electrode. Reversed-phase liquid chromatography (RPLC) together with a BDD electrode poised at +1.6 V vs. Pd/H2, was optimized for their sensitive detection. The separation was achieved in 60 s with a core-shell column (HALO C18). The detection limits of these analytes ranged from 10–30 nM. The method was applicable for the analysis of guaiacol and its precursors from a popular commercial drink. |
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| Keywords: | Alicyclobacillus spp Boron-doped diamond electrode Core-shell column Guaiacol High-performance liquid chromatography |
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