An Electrochemical Aptamer Biosensor for Bisphenol A on a Carbon Nanofibre-silver Nanoparticle Immobilisation Platform |
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| Authors: | Tebogo R. Tsekeli Luthando Tshwenya Tsholofelo I. Sebokolodi Thabile Ndlovu Omotayo A. Arotiba |
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| Affiliation: | 1. Department of Chemical Sciences, University of Johannesburg, Doornfontein, 2028 South Africa;2. Department of Chemistry, University of Eswatini, Kwaluseni, M201 Eswatini |
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| Abstract: | A nanocomposite platform of silver nanoparticles and carbon nanofibres (AgCNFs) was used to immobilise a bisphenol A specific 63-mer ssDNA aptamer to form a biosensor. The fabrication process of the biosensor was studied with electrochemical impedance spectroscopy and cyclic voltammetry in the presence of [Fe(CN)6]3−/4− as redox probe. The biosensor detected bisphenol A in a linear range of 0.1–10 nM, with a limit of detection of 0.39 nM using square wave voltammetry (SWV). The biosensor exhibited good selectivity in the presence of interfering species at 100-fold concentrations and was used to detect BPA in real water sample. |
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| Keywords: | Bisphenol A carbon nanofibres silver nanoparticles aptamer biosensor immobilisation platform |
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