Signal Amplification with Co(III) Protoporphyrin IX Nanoparticles and Anodic Stripping Voltammetry |
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| Authors: | Robert G Tzepos Easwer Raman Lauren E Toote David W Wright Aren E Gerdon |
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| Institution: | 1. Emmanuel College, Department of Chemistry and Physics, 400 Fenway, Boston, MA, 02115;2. Elizabethtown College, Department of Chemistry and Biochemistry, 1 Alpha Drive, Elizabethtown, PA, 17022;3. Vanderbilt University, Department of Chemistry, 7330 Stevenson Center, Nashville, TN, 37235 |
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| Abstract: | Electrochemical analysis of cobalt(III) protoporphyrin IX (CoP), synthesis and characterization of CoP nanoparticles, and signal amplification for biosensor development is presented. CoP was self-assembled into nanoparticles and then released to produce over 1000 electrochemically-detectable molecules for each protein target of interest, in this case monoclonal rabbit antibody. Anodic stripping voltammetry was utilized for quantitative and sensitive detection of CoP which correlated to target protein concentration. The CoP limit of detection was 4 nM and target protein was detected at 100 pM. This combination of nanoparticle and electrochemical signal amplification could allow for sensitive, inexpensive, and portable detection of protein biomarkers. |
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| Keywords: | porphyrinoids nanoparticles voltammetry biosensors |
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