Determination of complex type free,non-conjugated oligosaccharide glucose unit values in tomato xylem sap for early detection of nutrient deficiency |
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| Authors: | Bence Mátyás Júlia Singer Máté Szarka Daniel A. Lowy Boglárka Döncző Péter Makleit Virgilio E. Failoc-Rojas Andrés Ramirez Pedro Martínez Zsolt Sándor Ida Kincses András Guttman |
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| Affiliation: | 1. Genesis Sustainable Future Ltd., 33 Rákóczi St., Sárospatak, B-A-Z, H-3950 Hungary;2. Accelsiors Ltd., Budapest, Hungary;3. Horváth Csaba Memorial Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, Doctoral School of Molecular Medicine, University of Debrecen, Hungary;4. Institute for Nuclear Research (Atomki), Debrecen, Hungary;5. Faculty of Agricultural and Food Sciences and Environmental Management, University of Debrecen, Hungary;6. Unidad de Investigación para la Generación y Síntesis de Evidencias en Salud, Universidad San Ignacio de Loyola, Lima, Peru;7. Centro de Investigación y Transferencia de Tecnología - CIITT, Universidad Católica de Cuenca, Azogues, Ecuador;8. Research Group of Applied Plant Glycobiology, Dama Research Center limited, Kowloon, Hong Kong |
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| Abstract: | Although knowledge on glycan biosynthesis and processing is continuously maturing, there are still a limited number of studies that examine biological functions of N-glycan structures in plants, which remain virtually unknown. Here, the statistical correlation between nutrient (nitrogen) deficiency symptoms of crops and changes in 8-aminopyrene-1,3,6-trisulfonic acid (APTS)-labeled complex type free oligosaccharides is reported. While deficiency symptoms are predicted by multispectral images and Kjeldahl digestion, APTS-labeled complex type free oligosaccharides are identified by their glucose unit (GU) values in tomato xylem sap, using capillary electrophoresis with laser induced fluorescence detection (CE-LIF). Given the limited number of structures obtained from plants, archived in the literature, in the future, it is intended to create an open access database of promising indicators, namely, glycan structures that are presumably responsible for the nutrient deficiency caused stress in plants ( http://glycoplants.org ). |
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| Keywords: | Capillary electrophoresis with laser induced fluorescence detection (CE-LIF) Free oligosaccharides N-glycosylation in plants PCT-FNGs |
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