A Novel GABABR1a Receptor Electrochemical Biosensor Based on Gold Nanoparticles Chitosan-horseradish Peroxidase |
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Authors: | Xiaotong Zhao Lihui Wei Guangchang Pang Junbo Xie |
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Affiliation: | 1. Biotechnology & food Science College, Tianjin University of Commerce, Tianjin, 300134 PR China These authors contributed equally to this work.;2. Biotechnology & food Science College, Tianjin University of Commerce, Tianjin, 300134 PR China;3. School of Chinese Materia Medica, Tianjin University of Traditional Chinese Medicine, Tianjin, 301617 PR China |
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Abstract: | A novel gamma aminobutyric acid type B receptor subunit 1a (GABABR1a) biosensor modified with double-layer gold has been developed for the first time. In this work, the synthesized plasmid was transfected into HEK-293T cells by liposome transfection, and the target protein GABABR1a was obtained. The horseradish peroxidase was used as a signal amplification system to produce a double-layer gold modified GABABR1a receptor biosensor. The ligand gamma aminobutyric acid (GABA), jujuboside A and baclofen were detected by the time-current method with the biosensor. The results showed that the action regularity of GABA, jujuboside A, and baclofen on GABABR1a receptor was fitted with hyperbolic fitting (R2 was 0.9740, 0.9770 and 0.9770, respectively). The double reciprocal method was used to figure out the affinity constant (Ka) of GABA, jujuboside A, and baclofen with the receptor, i. e. 2.1016×10−15 mol/L, 1.7601×10−14 mol/L, and 1.633×10−14 mol/L, respectively, indicating that these measured compounds exhibited different action strength on the receptor. Based on the above, this study will provide a basis for drug screening and will also provide a new idea and method for investigating quantitatively the interaction between receptors and ligands in the future. |
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Keywords: | Nano biosensor GABABR1a Jujuboside A Baclofen GPCRs |
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