A Novel Molecule: 1-(2,6 Dichlorobenzyl)-4-(2-(2-4-hydroxybenzylidene)hydrazinyl)pyridinium Chloride and its Interaction with DNA |
| |
| Authors: | Ayça Karasakal Sülünay Parlar Vildan Alptüzün Arif E Cetin Seda Nur Topkaya |
| |
| Institution: | 1. Department of Chemistry, Faculty of Science and Letters, Namik Kemal University, Tekirdag, Turkey;2. Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Ege University, Izmir, Turkey;3. Izmir Biomedicine and Genome Center, Izmir, Turkey;4. Department of Analytical Chemistry, Faculty of Pharmacy, Izmir Katip Celebi University, 35620 Cigli, Izmir, Turkey |
| |
| Abstract: | Herein, a novel pyridine derivative, 1-(2,6 dichlorobenzyl)-4-(2-(2-4-hydroxybenzylidene)hydrazinyl)-pyridinium chloride (DHPC), was synthesized as a candidate drug molecule. Interaction of DHPC with DNA was used to explore its effect on DNA via Differential Pulse Voltammetry, Cyclic Voltammetry, and Electrochemical Impedance Spectroscopy. We demonstrated that oxidation signal of guanine bases of DNA decreased significantly while that of DHPC increased after its interaction with one another. Our candidate drug molecule exhibits LOD and LOQ, e.g., 1.5 μg/mL and 4.9 μg/mL, respectively. Toxicity effect value for DHPC (S%) was calculated as %31, demonstrating the candidate drug molecule's toxic effect on DNA. |
| |
| Keywords: | DNA drug molecule DNA and drug interaction pyridinium derivative electrochemical biosensor |
|
|
