Sensitive Electrochemical Determination of NADH Using an Electrode Fabricated by Intercalation of Tetrabutylammonium Ions Into Graphite Electrode |
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Authors: | Ozge Gorduk |
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Institution: | Department of Chemistry, Faculty of Arts and Science, Yildiz Technical University, TR34220 Istanbul, Turkey |
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Abstract: | In this study, we present a strategy for the specific electrochemical detection of NADH in human blood serums using modified electrode fabricated by intercalation of tetrabutylammonium ions (TBA+) into pencil graphite electrode (TBA+/PGE). In order to implement this protocol, a modification process in the potential range (?0.2) to (?2.75) V was performed in DMSO containing 0.1 M TBAP by CV method. The characterization processes of the prepared sensor were carried out using CV, EIS, FT-IR, XPS, and SEM methods. The electrode fabricated by intercalation of TBA+ was found to be able to analyze NADH at +0.32 V applied potential by the amperometric method. The LOD was detected as 0.46 μM. The analysis results of human blood serums which are taken from healthy individuals show that TBA+/PGE can be used for real samples. The results indicated that TBA+/PGE can be easily fabricated by one-step and one-pot electrochemical method and TBA+/PGE can be used as a potential sensor for the NADH determination. |
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Keywords: | Intercalation tetrabutylammonium perchlorate NADH non-enzymatic sensor amperometry |
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