| 蒸发光散射检测器HPLC法测定大豆低聚糖 |
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| 引用本文： | 夏海涛,刘玉芬,陈红卫. 蒸发光散射检测器HPLC法测定大豆低聚糖[J]. 应用化学, 2006, 23(4): 462-0 |
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| 作者姓名： | 夏海涛 刘玉芬 陈红卫 |
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| 作者单位： | 淮海工学院化学工程系；长春工业大学生物工程学院 |
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| 摘 要： | 蒸发光散射检测器HPLC法测定大豆低聚糖;HPLC;ELS检测器;梯度洗脱;低聚糖;大豆
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| 关 键 词： | 蒸发光散射检测器HPLC法测定大豆低聚糖 HPLC ELS检测器 梯度洗脱 低聚糖 大豆 |
| 文章编号： | 1000-0518(2006)04-0462-03 |
| 收稿时间： | 2005-07-03 |
| 修稿时间： | 2005-08-29 |
Analysis of Soybean Oilgosaccharides by HPLC with ELS Detector |
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| XIA Hai-Tao,LIU Yu-Fen,CHEN Hong-Wei. Analysis of Soybean Oilgosaccharides by HPLC with ELS Detector[J]. Chinese Journal of Applied Chemistry, 2006, 23(4): 462-0 |
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| Authors: | XIA Hai-Tao LIU Yu-Fen CHEN Hong-Wei |
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| Affiliation: | 1. Department of Chemical Engineering, Huaihai Institute of Technology, Lianyungang 222005 ;2. College of Bioengineering, Changchun University of Technology, Changchun |
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| Abstract: | Soybean oligosaccharides were analyzed via high performance liquid chromatography with gradient elution of acetonitril-water and an evaporative light-scattering detector. The three important components, sucrose, raffinose, stachyose of soybean within twenty minutes. The quantitative oilgosaccharides were separated completely from other components analysis results of the three components were achieved with the external standard method, and the linear relations were good. The recovery of this method is 96. 5% - 99. 1%, the relative standard deviations are in a range of 0. 18% -4.67%. Operation of the method is simple and fast, applicable to the routine analysis of industrial products. |
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| Keywords: | HPLC ELS detector gradient elution soybean oligosaccharide |
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