LC-MS/MS method for the quantification of myo- and chiro-inositol as the urinary biomarkers of insulin resistance in human urine |
| |
Authors: | Kim Byoung-Hyoun Park Jin-Young Jang Jae Bum Moon Dong Cheul |
| |
Institution: | Analytical Science, LG Chemical Research Park, LG Chemicals Ltd, 104-1 Moonji-Dong, Yusong-Gu, Daejeon 305-380, South Korea. |
| |
Abstract: | A simple LC‐MS/MS method has been developed and validated for the quantification of endogenous myo‐ and chiro‐inositol in human urine. myo‐ and chiro‐Inositol were completely resolved from other carbohydrates and there were no interference peaks in human urine. The correlation coefficient (n = 3) was greater than 0.9991 over the range 0.05–25.0 µg/mL with the weighted (1/C2) least square method. Precision (%RSD) and accuracy (%RE) were 0–10.0% and 0–6.0% for the intra‐day assay (n = 5) and 0–14.3% and 0–10.0% for the inter‐day assay (n = 5). myo‐ and chiro‐Inositol have been shown to be stable in human urine stored at room temperature and for three freeze–thaw cycles. Copyright © 2011 John Wiley & Sons, Ltd. |
| |
Keywords: | myo‐inositol chiro‐inositol urinary biomarker LC‐MS/MS method validation |
本文献已被 PubMed 等数据库收录! |
|