| CLEAVAGES OF TRANSCRIPTS OF TOBACCO MOSAIC VIRUS BY SYNTHETIC RIBOZYMES in vitro |
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| 作者姓名： | 许政皑 李洁 张庆琪 |
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| 作者单位： | National Laboratory of Plant Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，National Laboratory of Plant Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC，National Laboratory of Plant Molecular Genetics，Shanghai Institute of Plant Physiology，Academia Sinica，Shanghai 200032，PRC |
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| 基金项目： | Research suppordet by the Foundation of the President of the Chinese Academy of Sciences, the Foundation of Institute Directors of the Chinese Academy of Sciences and the Shanghai National Laboratory of Plant Genetics of the Chinese Academy of Sciences. |
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| 摘 要： | Based upon computer-assisted predictions on the secondary structures of tobacco mosaicvirus (TMV) genomic RNA (both polarities), hammerhead type ribozymes were synthesizedin vitro, which all shared a conserved domain adapted from satellite tobacco ringspot virus(sTobRV)RNA. Ribozymes RZ1, RZ2 and RZ3 were designed to cleave the phosphodiester bondsimmediate to the 3'--end of GUC between the residues 5384-5385 and 6312--6313 on the plusstrand and 1214-1215 on the minus strand, respectively. The in vitro data indicated that RZ1 wasable to cleave completely its substrates BT1(+ ) and BT2(+ ), representing partial sequencesof the plus strand of the TMV MP region at 50, 37 and 30℃ with a molar ratio of ribozymeto the target as low as 1:1. Its two iso-ribozymes RZ1A and RZ1B which were respectivelymodified to contain a CUUCGG sequence in the conserved region and in an additional 3'-ter-minal stem-loop of UUUUUCUUCGGAAAAA were able to cleave BT1(+) and BT2(+) asefficiently as RZ1. Ribozyme RZ3 cleaved, with l
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CLEAVAGES OF TRANSCRIPTS OF TOBACCO MOSAIC VIRUS BY SYNTHETIC RIBOZYMES in vitro |
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| XU ZHENG-KAI,LI JIE,ZHANG QING-QI National Laboratory of Plant Molecular Genetics.CLEAVAGES OF TRANSCRIPTS OF TOBACCO MOSAIC VIRUS BY SYNTHETIC RIBOZYMES in vitro[J].Science in China(Chemistry),1992(12). |
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| Authors: | XU ZHENG-KAI LI JIE ZHANG QING-QI National Laboratory of Plant Molecular Genetics |
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| Institution: | XU ZHENG-KAI,LI JIE,ZHANG QING-QI National Laboratory of Plant Molecular Genetics,Shanghai Institute of Plant Physiology,Academia Sinica,Shanghai 200032,PRC |
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