Generation of a hollow dark spherical spot by 4pi focusing of a radially polarized Laguerre-Gaussian beam |
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Authors: | Bokor Nándor Davidson Nir |
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Institution: | Department of Physics, Budapest University of Technology and Economics, Hungary. nandor.bokor@weizmann.ac.il |
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Abstract: | The properties of the focal spot for 4pi focusing with radially polarized first-order Laguerre-Gaussian beams are calculated. It is shown that a focal spot that has an extremely sharp dark region at the center and an almost-perfect spherical symmetry can be achieved. When such a hollow dark spherical spot is used in 4pi fluorescence depletion microscopy, an axial FWHM spot size of approximately 39 nm and a transverse FWHM spot size of approximately 64 nm can be achieved simultaneously in a practical system. |
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