Utilization of the IC-calorimeter for study of enzymatic reaction

In this article, the monitoring of an enzymatic reaction by means of a miniaturized batch type IC-calorimeter was performed. The aim of this work was focused on an investigation of enthalpy and rate of enzymatic reaction of trypsin with Nα-benzoyl-l-arginine-p-nitroanilide hydrochloride (BApNA). Both the parameters were determined for reactions in different buffers and for varying concentration of enzyme at 37 °C. The rate of reaction decreased with the increasing concentration of enzyme caused by trypsin autolysis.