Stepping towards highly flexible aptamers: enzymatic recognition studies of unlocked nucleic acid nucleotides |
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| Authors: | Dubois Camille Campbell Meghan A Edwards Stacey L Wengel Jesper Veedu Rakesh N |
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| Affiliation: | School of Chemistry and Molecular Biosciences, The University of Queensland, St Lucia, Brisbane, 4072, Australia. |
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| Abstract: | Enzymatic recognition of unlocked nucleic acid (UNA) nucleotides was successfully accomplished. Therminator DNA polymerase was found to be an efficient enzyme in primer extension reactions. Polymerase chain reaction (PCR) amplification of a 81 mer UNA-modified DNA library was efficiently achieved by KOD DNA polymerase. |
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