Portable capillary electrophoresis system with potential gradient detection for separation of DNA fragments |
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| Authors: | Xu Yan Qin Weidong Li Sam F Y |
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| Institution: | Department of Chemistry, National University of Singapore, Republic of Singapore. |
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| Abstract: | A portable capillary electrophoresis (CE) system with a novel potential gradient detection (PGD) was utilized to separate DNA fragments. For the first time it was demonstrated that separation of DNA fragments in polymer solution could be detected by a portable CE system integrated with PGD, with a limit of detection (LOD) comparable to that of the CE-ultraviolet (UV) method. Effects of buffer solution, sieving medium, and applied voltage were also investigated. The portable CE-PGD system shows several potential advantages, such as simplicity, cost effectiveness, and miniaturization. |
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| Keywords: | Capillary electrophoresis DNA fragment Potential gradient detection |
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