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Fundamental properties of isoelectric buffers for capillary zone electrophoresis |
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| Authors: | Alexander V. Stoyanov Pier Giorgio Righetti |
| Affiliation: | a Institute of Gene Biology, Russian Academy of Sciences, Vavilova Street, 34/5, 117334 Moscow B-334, Russia b University of Verona, Department of Agricultural and Industrial Biotechnologies, Strada Le Grazie, Cà Vignal, 37134 Verona, Italy |
| Abstract: | Different isoelectric buffers are analysed theoretically, taking into account a fundamental parameter, i.e., the ratio between intrinsic buffering power and conductivity (R=β/λ). For a model ampholyte, the above parameter is analysed both as a function of the pI and the pKb–pKa values. For natural pH gradients, the variation of R, connected with approaching the isoelectric point, is evaluated. A case of oligo-protic ampholytes is also considered. |
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