A Simple Method for Controlling the Degree of PEGylation of Dynorphin A (1-13) |
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| Authors: | WANG Xiufang TIAN Yong ZHONG Guoying CHEN Guanke |
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| Institution: | College of Pharmacy,Guangdong Pharmaceutical University,Guangzhou Higher Education Mega Center,Guangzhou,Guangdong 510006,China |
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| Abstract: | Dynorphin A (1–13) was chemically modified by reaction with succinimidyl propionate‐monomethoxy polyethylene glycol (mPEG‐SPA). To determine the degree and the optimized condition for PEGylation of dynorphin A, the reactions were monitored in different pH buffers at different molar ratios by reversed‐phase high performance liquid chromatography (RP‐HPLC) and matrix‐assisted laser desorption/ionization time‐of‐?ight mass spectrometry (MALDI‐TOF MS). The results showed that the degree of PEGylation for dynorphin A could easily be controlled through adjustment of the molar ratios and the pH. The degree of PEGylation of dynorphin A increased as the molar ratio of dynorphin to MPEG‐SPA and the pH increased. |
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| Keywords: | dynorphin A PEGylation degree molar ratio pH |
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