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Colorimetric Sensing of Adenosine Based on Aptamer Binding Inducing Gold Nanoparticle Aggregation
Authors:LIU  Xueping  ZHOU  Zhenhua  ZHANG  Liangliang  TAN  Zhongyang  SHEN  Guoli  YU  Ruqin
Institution:1. State Key Laboratory of Chem/Biosensing and Chemometrics,Chemistry and Chemical Engineering College,Hunan University,Changsha,Hunan 410082,China;Analytical and Testing Research Center,Henan University of Urban Construction,Pingdingshan,Henan 467044,China
2. State Key Laboratory of Chem/Biosensing and Chemometrics,Chemistry and Chemical Engineering College,Hunan University,Changsha,Hunan 410082,China
Abstract:A simple and rapid colorimetric approach for the determination of adenosine has been developed via target inducing aptamer structure switching, thus leading to Au colloidal solution aggregation. In the absence of the analytes, the aptamer/gold nanoparticle (Au NP) solution remained well dispersed under a given high ionic strength condition in that the random‐coil aptamer was readily wrapped on the surface of the Au NPs, which resulted in the enhancement of the repulsive force between the nanoparticles due to the high negative charge density of DNA molecules. While in the presence of adenosine, target‐aptamer complexes were formed and the conformation of the aptamer was changed to a folded structure which disfavored its adsorption on the Au NP surface, thus leading to the reduction of the negative charge density on each Au NP and then the reduced degree of electrostatic repulsion between Au nanoparticles. As a result, the aggregation of the Au colloidal solution occurred. The changes of the absorption spectrum could be easily monitored by a UV‐Vis spectrophotometer. A linear correlation exists between the ratio of the absorbance of the system at 522 to 700 nm (A522 nm/A700 nm) and the concentration of adenosine between 100 nmol·L?1 and 10 µmol·L?1, with a detection limit of 51.5 nmol·L?1.
Keywords:colorimetric sensing  modification-free Au NP aggregation  adenosine  aptamer  conformational change
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