Extraction, Purification and Anti-tumor Activity of Polysaccharide from Mycelium of Mutant Cordyceps Militaris |
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Authors: | ZHANG Ai-li LU Jia-hui ZHANG Na ZHENG Dan ZHANG Gui-rong and TENG Li-rong |
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Institution: | College of Life Science, Jilin University, Changchun 130012, P. R. China |
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Abstract: | A water-soluble polysaccharide(named MCMP) was isolated from the mycelium with high yield mutation Cordyceps militaris by hot-water extraction, deproteinization by sevage, alcohol precipitation, anion-exchange and gel filtration chromatography CL-6B. The polysaccharide contained mannose, rhamnose, galactose and glucose in a molar ratio of 59.36:1:8.31:39.50, of which the average molecular weight is 8100. In our research, Hep-G2 cells, Hela cells and mesangial cells were chosen to determine the anti-tumor activity of the polysaccharide. The results of MTT assay show that polysaccharides of the mutant strain presented inhibitory activity on the cells proliferation after 48 h incubation. |
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Keywords: | Cordyceps militaris Polysaccharide Anti-tumor |
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