首页 | 本学科首页   官方微博 | 高级检索  
     检索      

镉(Ⅱ)与牛血清白蛋白的相互作用及pH、钙(Ⅱ)、锌(Ⅱ)和表面活性剂的影响
引用本文:王夔,陈宝卫,李永昌,聂松青,王祥云,吴永慧.镉(Ⅱ)与牛血清白蛋白的相互作用及pH、钙(Ⅱ)、锌(Ⅱ)和表面活性剂的影响[J].无机化学学报,1985,1(1):40-51.
作者姓名:王夔  陈宝卫  李永昌  聂松青  王祥云  吴永慧
作者单位:北京医学院药学系,北京医学院药学系,北京医学院药学系,北京医学院基础医学系,北京大学技术物理系,北京大学技术物理系
基金项目:中国科学院科学基金资助课题
摘    要:本文用恢复电位法、荧光分光光度法及粘度法研究了镉(Ⅱ)与牛血清白蛋白(BSA)的配位平衡和pH的影响。结果表明BSA有两类镉结合部位(2个强结合和10个弱结合部位)。Cd—BSA配位反应受pH影响,在近中性pH范围Cd(Ⅱ)与BSA结合未接近饱和时,以CURFIT程序对结合曲线拟合发现,每摩尔BSA平均结合Cd(Ⅱ)摩尔数的对数值与游离镉浓度的对数间成线性关系,进一步用Marguardt法处理得到Cd(Ⅱ)与BSA结合平衡方程式:N =KCd2+]m,K和m均为pH函数。构象研究表明H+和Cd(Ⅱ)的结合都能引起BSA构象改变。用凝胶色谱法测定了Zn(Ⅱ)、Ca(Ⅱ)、吐温—80和十二烷基硫酸钠(SDS)对Cd—BSA结合的影响。Zn(Ⅱ)可以竞争Cd(Ⅱ)在BSA上结合部位使Cd(Ⅱ)活动化。SDS能够有效抑制Cd(Ⅱ)与BSA结合,这种作用可能与SDS引起BSA构象改变有关。

关 键 词:  牛血清白蛋白  表面活性剂
收稿时间:1983/11/28 0:00:00

INTERACTION BETWEEN CADMIUM (II) AND BOVINE SERUM ALBUMIN AND INFLUENCES OF pH, CALCIUM(II),ZINC(II) AND SURFACTANTS
Wang Kui,Chen Baowei,Li Rongchang,Nie Songqing,Wang Xiangyun and Wu Yonghui.INTERACTION BETWEEN CADMIUM (II) AND BOVINE SERUM ALBUMIN AND INFLUENCES OF pH, CALCIUM(II),ZINC(II) AND SURFACTANTS[J].Chinese Journal of Inorganic Chemistry,1985,1(1):40-51.
Authors:Wang Kui  Chen Baowei  Li Rongchang  Nie Songqing  Wang Xiangyun and Wu Yonghui
Institution:Department of Phaimacy, Beijing Medical College,Department of Phaimacy, Beijing Medical College,Department of Phaimacy, Beijing Medical College,Department of Basic Medical Sciences, Beijing Medical College,Department of Technical Physics, Beijing University and Department of Technical Physics, Beijing University
Abstract:The binding of cadmium ion to BSA and the pH effect Were studied by means of potential-restoration,fluorescence and viscosity studies. The results show that there may be two kinds of binding sites to cadmium (two strong and ten weak) .The binding of cadmium is significantly affected by variation of pH.In nearly neutral media,if Cd binding is not saturated,there is linear relation between the logarithm of number of moles of Cd bound per mole of BSA and the logarithm of free Cd ion concentration. Further treatment gives an equation N=KCd2+]m,with which the Cd -binding equilibrium may be estimated.In this equation,K and m are all functions of pH.The experimental results give information of the conforma-tional changes induced by the binding of proton or Cd(II). By means of gel chromatographic studies,the influences of Zn(II),Ca(II),Tween-80 and SDS were studied.The effective competition of Zn (II) may mobilize the BSA bound Cd(II).SDS inhibits the Cd-BSA binding.These actions may be relevant with conformation conversion.
Keywords:cadmium  bovine Serum albumin  surfactant
点击此处可从《无机化学学报》浏览原始摘要信息
点击此处可从《无机化学学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号