Surface planar bilayers of phospholipids used in protein membrane reconstitution: an atomic force microscopy study |
| |
Authors: | Doménech Oscar Merino-Montero Sandra Montero M Teresa Hernández-Borrell Jordi |
| |
Affiliation: | Departament de Química Física, UB E-08028-Barcelona, Spain. |
| |
Abstract: | In this work, using atomic force microscopy (AFM), we have studied the influence of the temperature on the properties of the surface planar bilayers (SPBs) formed with: (i) the total lipid extract of Escherichia coli; (ii) 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and 1,2-dimyristoyl-sn-glycero-3-phosphoethanolamine (DMPC) (1:1, mol/mol); and, 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanol-amine (POPE) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoglycerol (POPG) (3:1, mol/mol). According to the height profile analysis we performed, the height of the SPBs of DMPC:POPC were temperature dependent. Separated domains were observed in the SPBs of the POPE:POPG mixture and the E. coli lipid extract. The implication of those domains for the correct insertion of membrane proteins into proteoliposomes is discussed. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|