Development of Electroanalytical Methods Using Different Electrodes for the Determination of Progesterone (P4) in Pharmaceutical Formulations and Human,Cow and Goat Milk |
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| Authors: | Victoria Cruz Juan José Triviño Verónica Arancibia |
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| Institution: | 1. Pontificia Universidad Católica de Chile, Facultad de Química y de Farmacia, Santiago, 8331150 Chile;2. Universidad de Chile, Facultad de Ciencias Químicas y Farmacéuticas, Departamento de Química Inorgánica y Analítica, Santiago, 8380000 Chile |
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| Abstract: | For the determination of progesterone (P4) electroanalytical studies were performed using mercury electrode (HMDE) and screen-printed boron-doped diamond electrode (BDD-SPE). The effects of pH, supporting electrolyte, accumulation potential and time (Eacc, tacc) were studied in both systems. The optimum conditions were: i) HMDE: pH 7,0; Eacc: −0,1 V and tacc: 40 s, ii) BDD-SPE: 0,5 mol L−1 H2SO4; Eacc: 0,0 V and tacc: 120 s. Under these conditions, the detection limit was 3.1 μg L−1 for HMDE and 45.6 μg L−1 for BDD-SPE. Both methods were validated and applied in the P4 determination on pharmaceutical formulations and milk samples. |
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| Keywords: | Progesterone (P4) HMDE BDD-SPE Milk samples Pharmaceutical formulations (Progendo® Progest® Progeffik®) |
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