Collagen-based scaffolds enriched with glycosaminoglycans isolated from skin of Salmo salar fish |
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| Institution: | 1. Department of Chemistry of Biomaterials and Cosmetics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland;2. Department of Biology and Cell Imaging, Institute of Zoology and Biomedical Research, Faculty of Biology, Jagiellonian University in Kraków, Poland;1. National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu, China;2. Sichuan Testing Center for Biomaterials and Medical Devices, Sichuan University, 29 Wangjiang Road, Chengdu, China;1. Department of Neurobiology, Institute of Pharmacology, Polish Academy of Sciences, Smętna 12, 31-343 Kraków, Poland;2. Faculty of Chemistry, Jagiellonian University, ul. Ingardena 3, 30-060 Kraków, Poland;3. Department of Chemistry, Hankuk University of Foreign Studies, Yongin, Kyunggi-Do 449-791, Republic of Korea;4. Faculty of Foundry Engineering, AGH University of Science and Technology, ul. Reymonta 23, 30-059 Kraków, Poland;1. Laboratoire d’Amélioration des Plantes et Valorisation des Agro-Ressources, ENIS, Université de Sfax, 3038 Tunisia;2. Unité de Recherche Pharmacologie et Toxicologie des Xénobiotiques, Faculté de Médecine de Sfax, Sfax, 3029, Tunisia;3. Centre Régional de Transfusion Sanguine de Sfax, Route El-Ain Km 0.5, CP 3003 Sfax, Tunisia;4. Laboratoire Anatomie Pathologie, CHU Habib Bourguiba, Sfax 3029, Tunisia |
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| Abstract: | In this study both collagen and glycosaminoglycans were isolated from biodegradable waste. Namely collagen was isolated from rat tail tendons and glycosaminoglycans (GAGs) from fish skin. Porous materials were then obtained based on the isolated collagen with 1 or 5% addition of GAGs by freeze-drying process. The scaffolds were studied by infrared spectroscopy, mechanical testing and examined for the porosity and density. The scaffolds structure was observed by scanning electron microscope. The adhesion and proliferation of human osteosarcoma SaOS-2 cells was examined on prepared scaffolds to assess their biocompability.The results showed that the addition of glycosaminoglycans improves the properties of collagen-based scaffolds. Mechanical strength was increased by GAGs addition as well as the porosity of studied materials. Each scaffold with and without GAGs displayed porous structure with interconnected regular shaped pores. The attachment of cells was better for pure collagen scaffold, however, GAGs additive promoted the cells proliferation on the scaffold. |
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| Keywords: | Collagen Glycosaminoglycans Scaffolds Osteosarcoma SaOS-2 adhesion Tissue engineering |
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