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Electrochemical Generation of Hot Electrons in Fully Aqueous Solutions at Tetrahedral Amorphous Carbon Thin Film Electrodes and Electrochemiluminescence Immunoassay of Serum Amyloid A |
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| Authors: | Kalle Salminen Jian-Hui Fang Jarkko Etula Niklas Wester Jarkko Eskola Sakari Kulmala Jian-Jun Sun |
| Affiliation: | 1. Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350108 China;2. Department of Chemistry and Materials Science, Aalto University, Kemistintie 1, 02150 Espoo, Finland |
| Abstract: | Electrochemistry of hot electrons in fully aqueous solutions at tetrahedral amorphous carbon thin film electrodes is discussed. The generation of these highly reducing chemical species was confirmed by normal pulse voltammetry and several electrochemiluminescent systems. Electron transfer into pre-existing solvent cavities was observed at approximately −2.65 V vs. Ag/AgCl (sat.). Electrogenerated hot electrons were utilized as chemiluminescent mediators in heterogeneous sandwich immunoassay of Serum Amyloid A. The calibration curve was linear over four orders of magnitude and the detection limit was 85 ng L−1 that demonstrates the efficiency of hot electron generation at this electrode material. |
| Keywords: | Hydrated electrons Quasi-free electrons Electrochemiluminescence Bioaffinity assays |