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A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer-cDNA Structure |
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| Authors: | Elham Sameiyan Parirokh Lavaee Mohammad Ramezani Mona Alibolandi Zahra Khoshbin Prof Khalil Abnous Dr Seyed Mohammad Taghdisi |
| Institution: | 1. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Student Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran;2. Academic Center for Education, Culture and Research, Research Institute for Industrial Biotechnology, Industrial Biotechnology on Microorganisms, Mashhad, Iran;3. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran;4. Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran;5. Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran |
| Abstract: | In this study, a simple electrochemical sensing platform with the employment of a bivalent binding aptamer-cDNA probe (BBA-cDNA) structure is constructed for the detection of aflatoxin B1 (AFB1) as a mycotoxin. The BBA-cDNA structure is composed of two strands of aptamer (Apts) and their complementary strand (CS). Using a simple but accurate design, the presented measurement approach showed enhanced sensitivity and selectivity for AFB1 detection with a LOD of 0.1 ng/mL. The approach presented in this study can be applied to the development of biosensors for the measurement of various toxins by substituting the proper aptamers and complementary strands. |
| Keywords: | Electrochemical aptasensor Methylene blue Aflatoxin B1 BBA-cDNA structure |